abstract：:Human IgE antibodies from nine allergic subjects were found to react with poly-L-lysine (PLL) and other polyamines. Radioimmunoassay inhibition studies indicated that the two amino groups, but not the carboxyl, in lysine contributed to the antibody binding and 4-aminomethyl-1,8-octanediamine, a compound containing thr...

journal_title：Molecular immunology

authors： Zhao Z,Baldo BA,O'Brien RM,Plomley RF

更新日期：2000-04-01 00:00:00

abstract：:The transcription factor NF-kappaB plays critical roles in immune and inflammatory responses. Here we show that filarial parasitic sheath proteins cause activation of NF-kappaB in the airway epithelial HEp-2 cell line. This activation was transient and saturable, and involved degradation of the cytoplasmic inhibitor p...

journal_title：Molecular immunology

authors： Narayanan K,Balakrishnan A,Miyamoto S

更新日期：2000-02-01 00:00:00

abstract：:A variety of important cellular functions are regulated by cytokines. The Jak-STAT pathway is one of the important signaling pathways downstream of cytokine receptors. Following binding of a ligand to its cognate receptor, receptor-associated Jaks are activated. STAT proteins are then in turn activated by tyrosine pho...

journal_title：Molecular immunology

authors： Imada K,Leonard WJ

更新日期：2000-01-01 00:00:00

abstract：:The regulatory role of MEF2 (myocyte enhancer binding factor 2) proteins in nonmuscle tissues has not been well characterized. We examined the expression of MEF2 family members, namely, MEF2A, -B, -C, and -D, in the differentiation of HL60 promyeloid cells and observed the remarkable increase in the expressions of MEF...

journal_title：Molecular immunology

authors： Shin HH,Seoh JY,Chung HY,Choi SJ,Hahn MJ,Kang JS,Choi MS,Han TH

更新日期：1999-12-01 00:00:00

abstract：:It is widely believed that migrating immune cells utilise the intercellular junctions as routes of passage, and in doing so cause the transient disruption of junctional structures. Thus there is much interest in the molecules that have been identified at cell-cell contact points and their potential involvement in the ...

journal_title：Molecular immunology

authors： Williams LA,Martin-Padura I,Dejana E,Hogg N,Simmons DL

更新日期：1999-12-01 00:00:00

abstract：:One of the major obstacles in the successful clinical application of monoclonal antibodies has been the development of host immune responses to murine Ig constant and variable regions. While the CDR grafting of MAbs may alleviate many of these problems, the potential remains that one or more murine CDRs on the human I...

journal_title：Molecular immunology

authors： Iwahashi M,Milenic DE,Padlan EA,Bei R,Schlom J,Kashmiri SV

更新日期：1999-10-01 00:00:00

abstract：:A transplanted organ suffers inherently from an ischaemic insult and subsequent reperfusion injury. The severity of such early events is thought to influence the success of the transplant procedure, not only in the immediate post-transplant period, but also to predispose the graft to both acute and chronic rejection. ...

journal_title：Molecular immunology

authors： Dong J,Pratt JR,Smith RA,Dodd I,Sacks SH

更新日期：1999-09-01 00:00:00

abstract：:Humanization of rodent mAbs by CDR-grafting (also called "reshaping") is now a standard procedure for reducing immunogenicity and recruiting human effector functions. However, the design of the humanized mAb can sometimes prove circuitous. Attempts were made to humanize L-25, a mouse antibody against the human alpha-4...

journal_title：Molecular immunology

authors： Saldanha JW,Martin AC,Léger OJ

更新日期：1999-08-01 00:00:00

abstract：:Dynamic interactions between major histocompatibility complex (MHC) class I heavy chains and beta2-microglobulin (beta2m) play a critical role in their stability on the cell surface, and their ability to present peptide antigens to CD8+ T-cells. A cursory review of protein sequence homologies and three-dimensional cry...

journal_title：Molecular immunology

authors： Shields MJ,Hodgson W,Ribaudo RK

更新日期：1999-06-01 00:00:00

abstract：:During B and T lymphocyte development, immunoglobulin and T cell receptor genes are assembled from the germline V, (D) and J gene segments (Lewis, S.M., 1994. The mechanism of V(D)J joining: lessons from molecular, immunological and comparative analyses. Adv. Immunol. 56, 27-150). These DNA rearrangements, responsible...

journal_title：Molecular immunology

authors： Sun T,Ezekiel UR,Erskine L,Agulo R,Bozek G,Roth D,Storb U

更新日期：1999-06-01 00:00:00

abstract：:Invertebrate cells lack the p53 recombination checkpoint but contain mobile DNA sequences that transpose by a mechanism in part shared with excision of the V(D)J recombination signal sequences (RSS). In this work, inversion, deletion, and duplication of sequences associated with an invertebrate C. elegans Tc6 element ...

journal_title：Molecular immunology

authors： Dreyfus DH,Gelfand EW

更新日期：1999-05-01 00:00:00

abstract：:We have investigated the mechanism by which anti-CD28 antibodies activates IFN-gamma production by murine NK cells. These studies reveal that engagement of CD28 alone by this antibody is a poor activator of this cytokine response. Effective stimulation requires simultaneous ligation of the receptor for Fc (FcgammaRIII...

journal_title：Molecular immunology

authors： Cheung JC,Koh CY,Gordon BE,Wilder JA,Yuan D

更新日期：1999-04-01 00:00:00

abstract：:The xenotransplantation research is driven by the shortage of allografts for transplantation of patients with end-stage organ failure and by lack of success in developing suitable artificial organs. Pigs are now generally accepted to be the donor species of choice, although a possible risk for transmission of xenozoon...

journal_title：Molecular immunology

authors： Mollnes TE,Fiane AE

更新日期：1999-03-01 00:00:00

abstract：:The Pms2 gene is involved in DNA mismatch repair in mammalian cells, and has recently been shown to affect hypermutation of mammalian immunoglobulin genes. We have studied hypermutation of a lambda1 transgene in chronically stimulated Peyer's patch B cells of both young and old mice deficient in function of Pms2. In y...

journal_title：Molecular immunology

authors： Kong Q,Maizels N

更新日期：1999-02-01 00:00:00

abstract：:Der f 2 is one of the major mite allergens recognized by human IgE antibodies of allergic patients. Using five anti-Der f 2 mouse monoclonal antibodies, human IgE epitopes of Der f 2 were analyzed. Among them, two monoclonal antibodies 15E11 and 13A4 inhibited the binding between Der f 2 and human IgE antibodies. To d...

journal_title：Molecular immunology

authors： Nishiyama C,Hatanaka H,Ichikawa S,Fukada M,Akagawa-Chihara M,Yuuki T,Yokota T,Inagaki F,Okumura Y

更新日期：1999-01-01 00:00:00

abstract：:The architecture of lymphoid microenvironments depends upon complex interactions between several stromal cell types. We describe in this report the cloning of a cDNA which encodes a novel membrane molecule containing two external Ig-like domains. It is expressed at the junction between endothelial cells including HEV....

journal_title：Molecular immunology

authors： Malergue F,Galland F,Martin F,Mansuelle P,Aurrand-Lions M,Naquet P

更新日期：1998-12-01 00:00:00

abstract：:Phosphorothioate oligonucleotides (S-oligos) are nucleic acid derivatives that are commonly used as antisense agents. These compounds, similar to bacterial DNA and CpG oligonucleotides, display a variety of immunological activities in vitro and in vivo. To assess further these activities, the antigenicity of a series ...

journal_title：Molecular immunology

authors： Pisetsky DS,Reich CF 3rd

更新日期：1998-12-01 00:00:00

abstract：:The ubiquitin conjugating (ubc) E2 enzyme ubc-9 conjugates the ubiquitin-like peptide sentrin/SUMO-1/PIC1 to target proteins which include the Fas antigen. We show that the mouse genome contains four copies of the ubc-9 gene. These include a structural ubc-9 gene consisting of seven exons which encode a protein identi...

journal_title：Molecular immunology

authors： Tsytsykova AV,Tsitsikov EN,Wright DA,Futcher B,Geha RS

更新日期：1998-11-01 00:00:00

abstract：:In humans, three distinct but closely related classes of receptors that bind the Fc portion of IgG (FcgammaRI, II and III) have been identified. FcgammaRI can bind monomeric IgG with high affinity and has a unique third extracellular domain (EC3). Three very similar genes have been characterized for FcgammaRI (A, B, C...

journal_title：Molecular immunology

authors： Ernst LK,Duchemin AM,Miller KL,Anderson CL

更新日期：1998-10-01 00:00:00

abstract：:HCA2 is a widely used monoclonal antibody, thought to be highly selective for HLA-A and -G heavy chains. We demonstrate here that it also shows affinity to HLA-B73 and HLA-E molecules on intact cells. By comparing the differences in the amino acid (AA) sequences of several HLA class I alleles that are either recognise...

journal_title：Molecular immunology

authors： Seitz C,Uchanska-Ziegler B,Zank A,Ziegler A

更新日期：1998-09-01 00:00:00

abstract：:CD1 molecules are MHC-unlinked class Ib molecules consisting of classical (human CD 1a-c) and non-classical subsets (human CD1d and murine CD1). The characterization of non-classical subsets of CD1 is limited due to the lack of reagents. In this study, we have generated two new anti-mouse CD1 monoclonal antibodies, 3H...

journal_title：Molecular immunology

authors： Mandal M,Chen XR,Alegre ML,Chiu NM,Chen YH,Castaño AR,Wang CR

更新日期：1998-06-01 00:00:00

abstract：:The ragweed allergens Amb t 5 and Amb a 5 are among the smallest inhaled protein allergens known, containing a single, immunodominant T-cell epitope. In this study we analyzed the B-cell epitope structure of Amb t 5. The three-dimensional structures of Amb t 5 and Amb a 5 have been determined by NMR spectroscopy, prov...

journal_title：Molecular immunology

authors： Rafnar T,Brummet ME,Bassolino-Klimas D,Metzler WJ,Marsh DG

更新日期：1998-06-01 00:00:00

abstract：:Many T cell responses are dominated by restricted TCR expression and can range from repeated usage of particular TCR Vbeta- and/or Valpha-elements, to the preferential usage of both V- and J-elements, often in conjunction with conserved V-D-J or V-J junctional sequences. Cytotoxic T lymphocytes specific for a Kb-restr...

journal_title：Molecular immunology

authors： Turner SJ,Carbone FR

更新日期：1998-04-01 00:00:00

abstract：:Stimulation of human tonsillar mononuclear cells with interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) rapidly induced the activation of distinct nuclear factors with different mobilities, both of which bind the IL-4 response element (IL-4RE) of CD23b promoter as examined by electrophoretic mobility shift assays ...

journal_title：Molecular immunology

authors： Park HJ,So EY,Lee CE

更新日期：1998-03-01 00:00:00

abstract：:To clarify the different anti-CD26 mAbs corresponding different functions of CD26, the correlation of the epitopes defined by anti-CD26 mAbs and the functions of CD26 have been studied. Using truncated, human-rat CD26 swap mutants and cross-blocking studies, 13 anti-CD26 mAbs were divided into 5 separate groups. These...

journal_title：Molecular immunology

authors： Dong RP,Tachibana K,Hegen M,Scharpé S,Cho D,Schlossman SF,Morimoto C

更新日期：1998-01-01 00:00:00

abstract：:We previously showed that a variety of amino acid substitutions at positions 58 and 59 in the V(H) CDR2 of an anti-arsonate (Ars) antibody Fab simultaneously resulted in increased or unaltered affinity for Ars and substantially enhanced affinity for DNA. To test the generality of these observations, we generated and c...

journal_title：Molecular immunology

authors： Hande S,Manser T

更新日期：1997-12-01 00:00:00

abstract：:Forty different PCR clones encoding a llama variable heavy chain domain were analysed. The majority of these clones are derived from heavy-chain antibody cDNA in which the entire CH1 exon is absent. It appears from the amino acid within the VHH framework 1 and 3 that all the llama clones belong to the VH III family. H...

journal_title：Molecular immunology

authors： Vu KB,Ghahroudi MA,Wyns L,Muyldermans S

更新日期：1997-11-01 00:00:00

abstract：:We have determined the cDNA sequence encoding J chain, a polypeptide accessory molecule associated with polymeric Ig, from the anuran amphibian, Xenopus laevis (South African clawed frog). The translated polypeptide consists of 164 amino acid residues, including the signal sequence, and is somewhat longer than the cor...

journal_title：Molecular immunology

authors： Hohman VS,Stewart SE,Willett CE,Steiner LA

更新日期：1997-10-01 00:00:00

abstract：:Previously we reported that, early in the antibody response of BALB/c mice to several cytochromes c (CYT) coupled to ovalbumin (OVA), B cells responding to the self antigen mouse CYT recognized a single site on mouse CYT and were in much higher frequency than B cells responding to foreign CYT. In the present study the...

journal_title：Molecular immunology

authors： Mueller CM,Minnerath JM,Jemmerson R

更新日期：1997-08-01 00:00:00

abstract：:The recognition structure responsible for binding to conventional antigen on target cells has not previously been described for nonspecific cytotoxic cells (NCC) or for mammalian natural killer (NK) cells. Although several biochemical pathways may be available for initiation of the lytic cycle in NCC, evidence present...

journal_title：Molecular immunology

authors： Jaso-Friedmann L,Leary JH 3rd,Evans DL

更新日期：1997-08-01 00:00:00

abstract：:The interaction between a single-chain Fv (sFv) of the monoclonal antibody 3A21 and its antigen, bovine pancreatic ribonuclease A (RNase A), was studied by site-directed mutagenesis of the hypervariable regions and fluorescence polarization analysis. The affinity constants of wild-type sFv and a mutant sFv D31A (Asp31...

journal_title：Molecular immunology

authors： Katakura Y,Kumamoto T,Iwai Y,Kurokawa Y,Omasa T,Suga K

更新日期：1997-07-01 00:00:00

abstract：:Apo-1/Fas (CD95) is a transmembrane protein expressed on the cell surface that is involved in apoptosis and plays an important role in the function and regulation of the immune system. Aberrant expression of the Apo-1/Fas gene product has been reported in a number of immune-related disorders, such as autoimmune lympho...

journal_title：Molecular immunology

authors： Huang QR,Morris D,Manolios N

更新日期：1997-06-01 00:00:00

abstract：:Anti-human C1s monoclonal antibody H1532, a mouse gamma-1-immunoglobulin elicited by a C1r2C1s2 immunogen, appeared to bind to the beta-domain of C1s by electron microscopy. In agreement with this observation, Western blotting demonstrated good binding to unreduced C1s, but no binding to the alpha or gamma-B domains. ...

journal_title：Molecular immunology

authors： Tseng Y,Phillips ML,Schumaker VN

更新日期：1997-06-01 00:00:00

abstract：:Polyclonal or monoclonal human IgM rheumatoid factors (RF) react with eight antigenic sites on the CH3 IgG domain, four sites on CH2 and two on human beta 2-microglobulin. All 14 of these RF-reactive epitopes are linear 7-11 amino acid peptides with different primary sequence. We questioned whether RF reactivity with ...

journal_title：Molecular immunology

authors： Williams RC Jr,Malone CC,Kolaskar AS,Kulkarni-Kale U

更新日期：1997-05-01 00:00:00

abstract：:Previous work on the cis-acting elements that control heavy chain variable region (VH) gene somatic hypermutation has indicated the presence of an as yet unidentified element(s) 3' of the intron enhancer that is necessary for high rate mutation. Examination of cis-acting elements involved in kappa light chain V gene h...

journal_title：Molecular immunology

authors： Tumas-Brundage KM,Vora KA,Manser T

更新日期：1997-04-01 00:00:00

abstract：:Discordant xenografts surviving the initial hyperacute rejection phase may be subject to cellular rejection processes mediated by infiltrating leukocytes including T cells, NK cells and monocytes. The stable adhesion of these cell types to endothelial cells is due to the molecular interaction of the integrins VLA-4 an...

journal_title：Molecular immunology

authors： Mueller JP,Giannoni MA,Hartman SL,Elliott EA,Squinto SP,Matis LA,Evans MJ

更新日期：1997-04-01 00:00:00

abstract：:The human C epsilon gene produces a number of alternatively spliced heavy chain transcripts of which some encode functional IgE isoforms. We now show that differentially processed epsilon mRNA variants also exist in the mouse and are generated by differential polyadenylation and alternative splicing of primary epsilon...

journal_title：Molecular immunology

authors： Anand S,Batista FD,Tkach T,Efremov DG,Burrone OR

更新日期：1997-02-01 00:00:00

abstract：:We have designed and produced a stable bispecific scFv dimer (bisFv) by non-covalent association of two hybrid VH-VL pairs derived from an anti-neuraminidase antibody (NC10) and an anti-glycophorin antibody (1C3). The bisFv dimer was demonstrated to have binding activity to the two respective target antigens and was e...

journal_title：Molecular immunology

authors： Atwell JL,Pearce LA,Lah M,Gruen LC,Kortt AA,Hudson PJ

更新日期：1996-12-01 00:00:00

abstract：:Natural resistance to infection with intracellular parasites, such as Leishmania, Salmonella, and Mycobacterium, is controlled in mice by the expression of a single dominant gene locus designated Lsh/Ity/Bcg. Natural resistance-associated macrophage protein gene 1 (NRAMP1) was isolated as a candidate gene. NRAMP1 enco...

journal_title：Molecular immunology

authors： Kishi F,Yoshida T,Aiso S

更新日期：1996-11-01 00:00:00

abstract：:Multiple organ dysfunction syndrome (MODS) is a major cause of morbidity and mortality in surgical intensive care units. It is characterized by progressive failure of two or more organs remote from the origin of injury. Since MODS results from a severe generalized inflammatory response, both chemokines and complement ...

journal_title：Molecular immunology

authors： Miller CG,Cook DN,Kotwal GJ

更新日期：1996-10-01 00:00:00